Technical Laboratory Workshop: Antimicrobial Efficacy Test Methods and Activities February 18-19, 201

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Dates
Where
Registration
Summary
Agenda

Dates:

February 18, 2014, 8:30 a.m. to 5:00 p.m;

February 19, 2014, 8:30 a.m. to 5:00 p.m.

Where:

Environmental Protection Agency’s Environmental Science Center
701 Mapes Road
Ft. Meade, MD 20755-5350

Registration:

To register, go to cspa.org. For questions, contact Laura Geib (lgeib@cspa.org or 202-833-7320).

Space is very limited. Registration will be limited to one attendee per organization initially to ensure a broad cross-section of participants. Registration is limited to 15 participants on Day 1 and 50 participants on Day 2. The registration fee for Day 1 is $42; the fee for Day 2 is $32.

Summary

The U.S. EPA and Consumer Specialty Products Association invite interested stakeholders to participate in a laboratory-based technical workshop that will focus on several key test methods for evaluating the efficacy of disinfectants. The sessions will cover the efficacy testing of products with claims against spores of Clostridium difficile, the new Organization for Economic Cooperation and Development Quantitative Method for Evaluating Bactericidal Activity, the ASTM Quantitative Petri Dish method for evaluating the efficacy of antimicrobial wipes and other important methods related to efficacy requirements for registration of antimicrobials under the Federal Insecticide, Fungicide and Rodenticide Act. Demonstrations and training will be conducted in a laboratory setting. We will also discuss editorial and procedural revisions to other efficacy test methods such as the AOAC Use-dilution method. The results from the recent collaborative study with the OECD method (Mycobacterium terrae) and the Single Tube Method for biofilms will be presented. Implementation strategies for new and revised methods for performance testing of antimicrobials will also be provided. Stakeholders are expected to actively participate by providing relevant data and sharing their experiences working with these test methods.

Who Should Attend: This is a technical meeting for individuals with experience in conducting antimicrobial test methods or who plan to conduct these methods in the future. The agency is encouraging participation from a cross-section of affected parties including industry, federal and state partners and contract testing laboratories.

Participants must be knowledgeable of Biosafety Level II and III safety practices and procedures for working in a laboratory in order to participate in the laboratory demonstrations and training.

Agenda

2014 Technical Workshop on Antimicrobial Efficacy Test Methods and Activities (PDF) (3 pp, 74KB)

Tuesday, February 18th (AM Session) (830-1200) – Clostridium difficile, sporulation protocols and efficacy testing

Discussion Session #1 (830-915)

Introductions
Review of the Agenda
Lab safety practices and PPE
EPA’s Guidance on Testing C. difficile – an overview

C. difficile Lab Session #1 (930-1130)

Test Microbe

Strains
Diagnostic characteristics
Specialized equipment, media and reagents
Establishment of stock cultures

Sporulation protocol(s) – detailed demonstrations and review (ASTM E2839-11)
Spore purity and resistance testing – detailed demonstrations and review
Discussion of other acceptable sporulation protocols

Lunch (1200-100)

Tuesday, February 18th (PM Session) (100-500)

EPA Guidance for Clostridium difficile Testing (115-315)(PDF) (8 pp, 763 K)

Product efficacy testing – quantitative methodologies

ASTM 2197-11 (Quantitative Disk Carrier Test)– overview and demonstration
AOAC 2008.05 (Three Step Method)– overview and demonstration
ASTM 2896-12 (Quantitative Petri Plate) – potential for use and demonstration for wipes
Incubation and recording of results
Calculations and interpretation of results

Clostridium difficile: New Research Initiatives and Collaborative Study (330-500) (PDF) (29 pp, 2 MB)